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. Author manuscript; available in PMC: 2011 May 1.
Published in final edited form as: J Immunol. 2010 Mar 29;184(9):5029–5037. doi: 10.4049/jimmunol.0903463

Figure 6. let-7a and miR-125b directly target kB-Ras2 3′UTR.

Figure 6

(A) Alignment of let-7a and miR-125b with κB-Ras2 3′UTR. Solid lines indicate Watson-Crick base pairs. Dotted line indicates GU wobble pairs. The gray background denotes the seed binding region. (B) Secondary structure as predicted by RNAHybrid with κB-Ras2 3′UTR shown in black and miRNA in gray. (C) RAW 264.7 cells were transfected with luciferase expression vector containing the κB-Ras2 3′UTR or the control vector and synthetic pre-miR-125b, pre-let-7a or a negative control pre-miRNA. The cells were also transfected with a vector containing Renilla luciferase to serve as a transfection efficiency control. After 48 hours, cells were lysed and analyzed for luciferase expression using a dual-luciferase assay system. (D) PMA-differentiated U937 cells were transfected with pre-miR-125b, pre-let-7a or pre-miRNA negative control and analyzed for κB-Ras2 expression after 48 hours. *p<0.05 vs. negative control miRNA.

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