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. 2010 Jun 7;207(6):1173–1182. doi: 10.1084/jem.20091318

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© 2010 Morita et al.

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 3.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/3.0/).

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Figure 4. — Single-cell transplantation. (A–C) Single CD150^highCD34⁻KSL (A), CD150^medCD34⁻KSL (B), and CD150^negCD34⁻KSL cells (C) were transplanted into 40 lethally irradiated mice together with 2 × 10⁵ competitor cells. Blood of recipient mice was periodically analyzed 1, 2, 3, 4, and 5 mo after transplantation. Secondary transplantation was performed using 5 × 10⁶ reconstituted bone marrow cells of recipient mice. Blood of secondary-recipient mice was periodically analyzed 1, 2, 3, 4, and 5 mo after transplantation. Data from secondary transplantation show the mean percentage of chimerism (n = 3–5). The five sequential bars for each recipient mouse indicate the percentage of chimerism 1, 2, 3, 4, and 5 mo after transplantation. *, mice that died before secondary transplantation.