Figure 3.

Isolation of CD141⁺ DC and CD1c⁺ DC from apheresis products. (A) CD141⁺ DCs were enriched by an initial immunomagnetic depletion of lin⁺ cells and sorted as CD141^bright lin⁻CD1c⁻CD15⁻CD16⁻. (B) CD1c⁺ DCs were first enriched using a BDCA-1 immunomagnetic selection kit and further purified by flow cytometry sorting to remove residual contaminating cells. Morphology of purified DC is shown at 100× magnification. Bars, 10 µm.