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. 2010 Jun 7;207(6):1247–1260. doi: 10.1084/jem.20092140

Figure 5.

Figure 5.

CD141+ DCs secrete IFN-β, CXCL10, and IL-12p70. (A) Expression of costimulatory molecules CD40, CD80, CD83, and CD86 by CD141+ DC and autologous CD1c+ DC freshly isolated (0 h) or after 24-h culture in complete medium alone (24 h) or in the presence of poly I:C (24 h PIC). Left, one example of three donors is shown in histograms. Right, CD141+ DCs express higher levels of costimulatory markers after activation with poly I:C compared with autologous CD1c+ DCs. The fold increase in the MFI over the isotype control for three donors is shown. (B) Secretion of inflammatory cytokines and chemokines by CD141+ DC and autologous CD1c+ DC after stimulation with poly I:C and secretion of IL-12p70 after activation with a cocktail of poly I:C, IFN-γ, TNF, IFN-α, and IL-1β.