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. 2010 Jun 7;207(6):1209–1221. doi: 10.1084/jem.20091299

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© 2010 Duy et al.

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Figure 5. — BCL6 is required for the development of a diverse primary B cell repertoire. (A) For two pairs of BCL6^+/+ and BCL6^−/− mice (I and II), in addition to bone marrow samples spleen tissue was also available. Immature B cells (fraction E) from bone marrow and mature B cells from spleens (fraction F) were sorted and subjected to spectratyping analysis to study length diversity of V_H-DJ_H junctions. In two BCL6^−/− mice, clonal expansions were identified that occurred both in the bone marrow and in the spleen with the same peak size, i.e., the same length of the V_H-DJ_H junction. Two such clonal expansions were found in BCL6^−/− mouse I (I.1 and I.2) and one expansion was found in BCL6^−/− mouse II (II.1). (B) To test whether these expansions with the same length of the V_H-DJ_H junction indeed belong to one clone, we performed a detailed sequence analysis of these V_H-DJ_H junctions. Alignments of V_H, D, and J_H gene segments are shown as well as N/P nucleotides in the junctional regions. Internal heptamers are highlighted in red, and sites at which the junctional homology with the parental V_H-DJ_H rearrangement ends are indicated by arrows.