Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2010 Jun 7;207(6):1145–1152. doi: 10.1084/jem.20091245

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2010 Westerberg et al.

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 3.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/3.0/).

PMC Copyright notice

Figure 3. — Normal migratory response but reduced spreading of WASP-L272P and WASP-I296T B and T cells. (A) Migration. Spleen B or T cells were allowed to migrate to CXCL12 or CCL19 for 3 h using an in vitro chemotaxis chamber. Migrating cells were collected and enumerated by flow cytometry with reference beads. The percentage of migrating cells is shown as mean values (±SD) of triplicate wells and is representative of at least three experiments. *, P < 0.05 compared with WT. (B) Spreading. (top) Spreading of T cells was assessed on anti-CD3 plus anti-CD28 antibody–coated surfaces. Closed white arrows depict the formation of large sheet-like lamellipodia of spread T cells. Bars, 25 µm. (bottom) Spreading of activated B cells was assessed on anti-CD44 antibody–coated surfaces. Closed white arrows depict the formation of long protrusions of spread B cells and open white arrows depict spread cells with many short protrusions. Bars, 40 µm. (C) Graphs show the mean of relative numbers (±SD) of spread T (left) and B cells (right) in triplicates and are representative of three experiments. *, P < 0.05 compared with WT.