Figure 5.

DNGR-1⁺ BDCA3⁺ DCs respond to poly I:C and to TLR8, but not TLR7 agonists. (A) Purified DNGR-1⁺ BDCA3⁺ DCs from CBDCs (DNGR-1⁺ CBDCs) or from humanized mice (DNGR-1⁺ hu mice), or BDCA2⁺ pDCs from the same humanized mice (BDCA2⁺ hu mice), were cultured with poly I:C (10 µg/ml), imiquimod (10 µg/ml), or medium alone. After overnight incubation, culture supernatant was tested for TNF and IL-6 content. (B) DNGR-1⁺ CBDCs as in (A) were cultured with resiquimod (10 µg/ml), poly U (10 µg/ml) or medium alone. Supernatant was tested for IL-6 after overnight culture. (C) DNGR-1⁺ BDCA3⁺ DCs purified from CBDCs (DNGR-1⁺ CBDCs) or human spleen (DNGR-1⁺ hu spleen) were cultured with a mix of TLR agonists and cytokines with or without antigen-specific T cells and antigen, as described in the Materials and methods. Supernatant was tested for IL-12 p70 after overnight culture. Data in A–C are representative of at least two independent experiments with independent sources of cells. ND, not detectable.