FIG. 5.

Additional sites of GFP fluorescence in Tg(Eomes::GFP) embryos at midgestation to fetal stages of development. (a,b) A population of fluorescent cells present in the placenta of E14.5 embryos. (c–e) Rendered confocal images depicting nuclear counterstain (c), GFP (d), and two channel merge (e). (f) Highmagnification rendered image (boxed region in e) showing cell morphology and localization of GFP-positive cells. (g) Brightfield and GFP overlay showing fluorescence in the pylorus and pyloric sphincter region of the stomach. (h–k) Rendered confocal images of nuclei (h), GFP (i), F-actin (j), and three channel merge (k). (l) High magnification image depicting the elongated cell morphology of GFP-positive cells. (m) Brightfield andGFP overlay ofGFP fluorescence in the developing pancreas. (n–q) Rendered confocal images of nuclei (n),GFP (o), F-actin (p), andmerge (q). (r) High magnification image showing localization and morphology of GFP-positive cells. (s–u) Rendered confocal images of GFP (s), E-cadherin (t), and two channel merge with Hoechst nuclear counterstain (depicted in blue) (u), within the pylorus of the stomach. High magnification inset demonstrating that GFP positive cells, representing a subpopulation of cells within the mucosa, are part of an epithelium and stain positive for E-cadherin. (v–x) Rendered confocal images of GFP (v), Pdx-1 (pancreatic duodenum homeobox-1) (w), and two channel merge with Hoechst nuclear counterstain (x). High magnification inset illustrating that GFP fluorescence does not colocalize with differentiating Pdx1-positive pancreatic beta cells. Scale bars = 100 μm. umb, site of attachment of the umbilicus; lab, labyrinthine layer; sp, spongiotrophoblast;mp,muscularis propria; sm, submucosa;m,mucosa.