Table 1.
Differential macrophage gene expression in M. avium 109 and 2D6 mutant
| Gene | Gene Bank ID | Name | Function | Fold induction (± SD) | p value <0.05 |
|---|---|---|---|---|---|
| APBA2 | AB014719 | Amyloid beta (A4) precursor protein binding | Signal transduction | 10.7 ± 2.3 | Y |
| CYP4F2 | U02388 | Cytochrome P450 | Inactivation & degradation of leukotriene B4 | 2.6 ± 0.9 | Y |
| DGKA | AF064767 | Diacylglycerol kinase alpha | Intracellular signaling | 2.3 ± 0.4 | Y |
| DGKD | D63479 | Diacylglycerol kinase delta | Phosphatidylinositol signaling | 6.7 ± 1.2 | Y |
| DYNC1H1 | AB002323 | Cytosolic dyenin heavy chain | Microtubule reorganization | 17.4 ± 3.1 | Y |
| GPD2 | NM_000408 | Glycerol-3-phosphate dehydrogenase 2 | Glycerol-3-phosphate metabolism | 3.5 ± 0.4 | Y |
| GRK4 | NM_005307 | G-protein coupled receptor kinase 4 | Regulation of G-protein coupled receptor protein signaling | 3.5 ± 0.6 | Y |
| HIPK3 | AF004849 | Homeodomain interacting protein kinase 3 | Inhibition of apoptosis | 2.05 ± 0.3 | Y |
| INPP1 | NM_002194 | Inositol polyphosphate-1-phosphatase | Phosphatidylinositol signaling | 2.0 ± 0.4 | Y |
| ITK | D13720 | IL2-inducible T-cell kinase | T-cell proliferation & differentiation | 2.4 ± 0.4 | Y |
| LCK | M36881 | Lymphocyte-specific protein tyrosine kinase | Intracellular signaling | 3.5 ± 0.7 | Y |
| NFKB1 | M58603 | Nuclear factor of kappa light polypeptide gene enhancer in B-cells 1 | Transcriptional regulator | 2.3 ± 0.4 | Y |
| PDE1C | U40371 | Calcium/calmodulin-dependant 3', 5'-cyclic nucleotide phosphodiesterase 1C | Signal transduction | 17.4 ± 1.9 | Y |
| PKIA | S76965 | Protein kinase (cAmp-dependent) inhibitor alpha | Negative regulation of protein kinase A | 2.0 ± 0.3 | Y |
| PPM1G | Y13936 | Serine/threonine protein phosphatase PP1-gamma 1 catalytic subunit | Negative regulator of cell stress response/cell cycle arrest | 3.2 ± 0.5 | Y |
| PTPN11 | D13540 | Protein tyrosine phosphatase | Intracellular signaling, cell migration | 2.4 ± 0.2 | Y |
| RGS3 | AF006610 | Regulator of G-protein signaling-3 | Inhibition of G-protein mediated signal transduction | 3.4 ± 0.3 | Y |
| RORC | U16997 | RAR-related orphan receptor C | Inhibition of Fas ligand and IL2 expression | 3.1 ± 0.3 | Y |
| ROR1 | M97675 | Receptor tyrosine kinase-like orphan receptor 1 | Unknown | 4.0 ± 0.4 | Y |
Complemented 2D6 mutant had similar results to the wild-type bacterium. Y = Yes; N = No