Table 2.
Macrophage genes with decreased expression in M. avium 109 but increased in 2D6 mutant 4 h post infection
| Gene | Gene Bank ID | Name | Function | Fold induction (± SD) | p value <0.05 |
|---|---|---|---|---|---|
| AMBP | X04494 | Alpha-1-microglobulin | Negative regulation of immune response/Protease inhibitor | 4.2 ± 0.7 | Y |
| BLK | BC004473 | B-lymphoid tyrosine kinase | Apoptosis | 3.3 ± 0.3 | Y |
| BMX | AF045459 | BMX non-receptor tyrosine kinase | Intracellular signaling | 18.6 ± 4.1 | Y |
| CCR3 | AF247361 | Chemokine receptor 3 | Signal transduction | 4.1 ± 0.6 | Y |
| CD53 | BC040693 | CD53 molecule | Growth regulation | 4.1 ± 0.3 | Y |
| CETN2 | X72964 | Centrin, EF-hand protein 2 | Microtubule organization center | 6.3 ± 0.9 | Y |
| CHP | NP_009167 | Calcium binding protein P22 | Potassium channel regulator/Signal transduction | 20.8 ± 3.5 | Y |
| CR1 | Y00816 | Complement receptor 1 | Bacterial uptake | 4.3 ± 0.4 | Y |
| CTSG | NM_001911 | Cathepsin G | Bacterial killing | 2.9 ± 0.2 | Y |
| DCTN1 | NM_004082 | Dynactin 1 | Lysosome and endosome movement | 35.8 ± 8.0 | Y |
| DDOST | D29643 | Dolichyl-diphosphooligosaccharide-protein glycosyltransferase | N-linked glycosylation | 3.3 ± 0.3 | Y |
| DGKG | AF020945 | Diacylglycerol kinase gamma | Intracellular signaling | 5.3 ± 0.6 | Y |
| DGKZ | U51477 | Diacylglycerol kinase zeta | Intracellular signaling | 48.1 ± 6.1 | Y |
| EMILIN2 | AF270513 | Elastin microfibril interfacer 2 | Cell adhesion | 14.7 ± 3.7 | Y |
| FOXC2 | Y08223 | Forkhead box C2 | Transcription factor | 5.9 ± 1.5 | Y |
| GABBR1 | Y11044 | GABA receptor 1 | Signal transduction | 6.1 ± 2.0 | Y |
| GABBR2 | AF069755 | GABA receptor 2 | Signal transduction | 2.8 ± 0.4 | Y |
| GPR17 | NM_005291 | G-protein coupled receptor 17 | Signal transduction | 83.2 ± 12.5 | Y |
| GZMA | NM_006144 | Granzyme A | Apoptosis | 2.1 ± 0.6 | Y |
| IFNA1 | NM_024013 | Interferon alpha 1 | Intracellular signaling | 2.6 ± 1.0 | Y |
| IL10RA | U00672 | Interleukin 10 receptor alpha | Inhibition of proinflammatory cytokine synthesis | 2.3 ± 0.2 | Y |
| ITGB1 | BC020057 | Fibronectin receptor | Bacterial uptake | 4.6 ± 0.7 | Y |
| LCP2 | NM_005565 | Lymphocyte cytosolic protein 2 | Immune response | 37.5 ± 9.2 | Y |
| MCAM | X68264 | Melanoma cell adhesion molecule | Cell adhesion | 4.7 ± 0.2 | Y |
| MS4A1 | M27394 | Membrane-spanning 4-dmains | Immune response | 9.6 ± 0.9 | Y |
| PBX2 | NM_002586 | Pre-B-cell leukemia transcription factor 2 | Transcriptional activator | 3.0 ± 0.3 | Y |
| PLTP | NM_006227 | Phospholipid transfer protein | Lipid metabolism | 3.6 ± 0.5 | Y |
| RAB7 | X93499 | RAS related GTP binding protein | Vesicular transport regulation | 3.4 ± 0.4 | Y |
| RAB13 | X75593 | RAS related GTP binding protein | Small GTPase mediated signal transduction | 7.5 ± 1.1 | Y |
| RGS12 | AF035152 | Regulator of G-protein signaling 12 | Negative regulation of G-protein signaling | 3.0 ± 0.3 | Y |
| RGS13 | AF030107 | Regulator of G-protein signaling 13 | Negative regulation of G-protein signaling | 2.6 ± 0.4 | Y |
| S100A11 | NM_005620 | Calgizzarin | Motility, invasion & tubulin polymerization | 9.6 ± 0.8 | Y |
| TNFRSF17 | Z29574 | TNF receptor | Immune response | 2.6 ± 0.3 | Y |
| TUBB | AB062393 | Tubulin beta | Microtubule based movement | 4.0 ± 0.3 | Y |
| YWHAZ | NM_003406 | Tyrosine 3-monooxygenase | Signal transduction | 4.3 ± 0.5 | Y |
Complemented 2D6 mutant had similar results to the wild-type bacterium. Y = Yes; N = No