Table 3.
List of phagosomal proteins identified by MS/MS post-infection with MAC 109 or 2D6 at different time points
| MAC 109 | 2D6 mutant | ||||||
|---|---|---|---|---|---|---|---|
| Protein | Accession | (h) | (h) | ||||
| Bacterial Uptake | number | 0.5 | 4 | 24 | 0.5 | 4 | 24 |
| Complement c1q tumor necrosis factor related protein 5 | Q9BXJ0 | x | x | x | |||
| Complement receptor 2 | P20023 | x | |||||
| Macrophage receptor MARCO | Q9UEW3 | x | x | x | |||
| Pulmonary surfactant protein D | P35247 | x | |||||
| Scavenger receptor with C type Lectin type 1 | Q9BYH7 | x | |||||
| TNFRSF1A-associated via death domain/TRADD | Q15628 | x | |||||
| Tumor necrosis factor receptor member 1A/TNFRSF1A | 1NCFA | x | |||||
| Antigen Presentation & Recognition | |||||||
| Integrin alpha 1 | P56199 | x | |||||
| Integrin alpha IIb | P08514 | x | |||||
| MHC class I | Q95ID4 | x | |||||
| MHC class II | I54427 | x | |||||
| Rab Interacting | |||||||
| EEA1 | Q15075 | x | |||||
| Peroxin 5-related protein | Q81YB4 | x | x | ||||
| Rabaptin 5 | Q15276 | x | |||||
| Cytoskeletal Proteins & Motors | |||||||
| Alpha actin | P62736 | x | |||||
| Ankyrin 1 | Q6S8J3 | x | |||||
| Beta actin | Q96B34 | x | x | ||||
| E-MAP-115 | Q14244 | x | |||||
| Keratin, type II cytoskeletal I | P04264 | x | x | ||||
| Kinesin/KIF3 | O14782 | x | |||||
| Kinesin family member 26A | Q8TAZ7 | x | |||||
| L-plastin | P13796 | x | x | ||||
| Myosin heavy chain non-muscle type A | P35579 | x | |||||
| Myosin X with ATPase activity | Q9HD67 | x | x | ||||
| RHOC | P08134 | x | |||||
| TCP-1 zeta | P40227 | x | |||||
| Tubulin alpha | Q71U36 | x | x | ||||
| Tubulin alpha 2 | Q13748 | x | |||||
| Tubulin alpha 3 | Q71U36 | x | |||||
| Tubulin beta 2 | P07437 | x | x | ||||
| Proteins in Biosynthetic Pathways | |||||||
| ABC transporter 2 | Q9BZC7 | x | |||||
| ADP-ATP translocase | P05141 | x | |||||
| Aldoketo-reductase 3 | Q9UFE1 | x | |||||
| ALG12 | Q9BV10 | x | |||||
| ATP synthase | P06576 | x | x | ||||
| Fatty acid synthase | P49327 | x | x | ||||
| Fatty acid transporter member 1 | Q6PCB7 | x | |||||
| GAPDH | P04406 | x | |||||
| Phosphatidyl inositol glycan class T | Q92643 | x | |||||
| IMP dehydrogenase | P12268 | x | |||||
| K-Cl co-transporter | T17231 | x | |||||
| Mitochondrial dicarboxylate carrier | Q9UBX3 | x | |||||
| Neutral amino acid transporter | Q15758 | x | |||||
| NUAK family, SNF1-like kinase 1 | O60285 | x | |||||
| PI4KII | Q9BTU6 | x | |||||
| Pyruvate kinase | PS00110 | x | x | x | |||
| Ribophorin II | Q5JYR7 | x | |||||
| Trehalose precursor | O43280 | x | |||||
| Transcriptional Regulators | |||||||
| Lysine-specific histone demethylase 1 | O60341 | x | |||||
| CRSP complex subunit 2 | O60244 | x | |||||
| CREB binding protein | Q75MY6 | x | |||||
| DEAH box polypeptide 9 | Q08211 | x | |||||
| IFI 16 | Q16666 | x | |||||
| Msx2-interacting protein | Q96T58 | x | |||||
| p-100 co-activator/SND1 | Q7KZF4 | x | |||||
| p-300/CBP associated factor | Q92831 | x | |||||
| Zinc finger & BTB domain containing protein 4 | Q9P1Z0 | x | |||||
| Zinc finger protein 588 | Q9UII5 | x | |||||
| Zinc finger protein 43 | P17038 | x | |||||
| 60 S acidic ribosomal protein P2 | P05387 | x | |||||
| 60 S ribosomal protein L6 | Q02878 | x | x | ||||
| 60 S ribosomal protein L9 | P32969 | x | |||||
| 60 S ribosomal protein L14 | P50914 | x | |||||
| Proteins Interacting with Signal Proteins | |||||||
| cAMP-specific 3',5'-cyclic phospho-diesterase | Q08493 | x | |||||
| Calcium & lipid binding protein/NLF2 | 388125 | x | |||||
| Chondroitin sulfate synthase 3 | Q86Y52 | x | |||||
| CXC3C membrane-associated chemokine | P78423 | x | |||||
| Doublecortin & CaM kinase like-3 | Q9C098 | x | |||||
| Dystrobrevin alpha | Q9Y4J8 | x | |||||
| Golgin subfamily A member 5 | Q8TBA6 | x | |||||
| Microtubule associated-Ser/The kinase 3 | O60307 | x | |||||
| Protein kinase A anchoring protein 9 | Q99996 | x | |||||
| Protein kinase N | Q8NF44 | x | |||||
| Serine/theonine kinase 16 | Q5U0F8 | x | |||||
| TER ATPase | P55072 | x | |||||
| Ion Channels | |||||||
| Amiloride-sensitive cation channel | Q96FT7 | x | |||||
| Voltage dependent-N-type calcium channel alpha 1B subunit | Q00975 | x | |||||
| Voltage dependent-T-type calcium channel alpha 1I subunit | Q9P0X4 | x | |||||
| Other Proteins | |||||||
| AFG3 like protein 2 | Q9Y4W6 | x | |||||
| APBB1 | O00213 | x | |||||
| Astrotactin 2 | O75129 | x | |||||
| Apoptotic chromatin-condensation inducer in the nucleus | Q9UKV3 | x | |||||
| Clathrin heavy chain 1 | Q00610 | x | |||||
| Ephrin B3 | Q15768 | x | |||||
| 48 kda histamine receptor subunit peptide 4 | AAB34251 | x | |||||
| HSP 60 | P10809 | x | |||||
| HSP 90 | P07900 | x | |||||
| Importin alpha 2 | P52292 | x | |||||
| Interferon regulatory factor 6 | O14896 | x | |||||
| LRCH4/Ligand binding receptor | O75427 | x | |||||
| NADPH oxidase activator 1 | Q2TAM1 | x | |||||
| Protein disulfide-isomerase A6 | P07237 | x | x | ||||
| p-53-associated parkin-like cytoplasmic protein/PARC | Q8IWT3 | x | |||||
| Serine protease inhibitor | Q9NQ38 | x | |||||
| Vitrin | Q6UX17 | x | |||||
| 14-3-3 protein/Tyrosine 3-monoxygenase | P62258 | x | |||||
| Proteins with Unknown Function | |||||||
| Hypothetical protein DKFZp434A128 | T34567 | x | |||||
| Hypothetical protein LOC136288 | Q8NEG2 | x | |||||
| Hypothetical protein KIA1783 | Q96JP2 | x | |||||
| Hypothetical protein KIAA1783 | Q96JP2 | x | |||||
| Hypothetical protein FLJ32795 | Q96M63 | x | |||||
| Hypothetical protein FLJ42875 | Q8N6L5 | x | |||||
| Hypothetical protein FLJ45491 | Q6ZSI8 | x | |||||
| Hypothetical protein DKFZp434G131 | Q9H0H4 | x | |||||
| Hypothetical protein FLJ46534 | Q6ZR97 | x | |||||
| Hypothetical protein FLJ00361 | Q8NF40 | x | |||||
Complemented 2D6 mutant had similar results to the wild-type bacterium. Y = Yes; N = No