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Sense and antisense primers for real-time PCR
| Target | Primers | PCR product (bp) |
|---|---|---|
| β-actin | 5'-TGATGGTGGGCATGGGTCAGA-3' 5'-CCCATGCCAATCTCATCTTGT-3' |
800 |
| GRK4 | 5'-AATGTATGCCTGCAAAAAGC-3' 5'-GATTGCCCAGGTTGTAAATG-3' |
235 |
| DGKD | 5'-CTCGGCTTACGGTTATTCCAG-3' 5'-CCATCTCCATCTTCAGCCTCC-3' |
656 |
| LCP2 | 5'-CACTGAGGAATGTGCCCTTTC-3' 5'-GTGCCTCTTCCTCCTCATTGG-3' |
408 |
Complemented 2D6 mutant had similar results to the wild-type bacterium. Y = Yes; N = No
