Table 3. Confirmation of selected genes using RT-PCR.

Encoded Protein/Gene	Symbol	Microarray			RT-PCR
		APAP	IFN-β	IFN-β+APAP	APAP	IFN-β	IFN-β+APAP
Heme oxygenase (decycling) 1	Hmox1	2.7	−1.5	2.3	9.6	−3.8	12.1
Keratin 18	Krt18	−1.3	1.1	−2.0	−1.7	1.1	−2.0
B-cell linker	Blnk	−1.0	2.6	1.2	−2.3	10.1	6.1
2′–5′ Oligoadenylate synthetase 2	Oas2	1.0	1.8	1.3	−1.3	26.8	13.0
Interleukin-1 receptor-associated kinase 2	Irak2	−1.1	−1.0	−1.6	−1.5	−2.8	−4.0
EG634650	EG	−1.2	6.5	4.1	−3.6	95.3	24.7
Interferon-induced protein with tetratricopeptide repeats 3	Ifit3	−1.1	49.6	29.9	−3.1	376.9	109.2
Interferon Stimulated Gene 15 ubiquitin-like modifier	Isg15	−1.3	20.0	12.3	−1.2	245.1	96.1
2′–5′ Oligoadenylate synthetase-like 1	Oasl1	−1.2	13.3	7.3	−1.8	298.0	80.6
Z-DNA binding protein 1	Zbp1	−1.2	13.1	6.7	−2.6	113.5	71.3

The absolute fold-change was determined using semi-quantitative RT-PCR on 5 genes from the 4 hour time point and compared to the microarray analysis. In all cases numbers are relative to vehicle control.