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. 2010 Jun 9;5(6):e11039. doi: 10.1371/journal.pone.0011039

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Kizilyaprak et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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(A) Table showing the labelling densities found for each antibody in the different chromatin compartments. (B) Graph representing the labelling density of the silencing histone marks in concentric nuclear compartments. From left to right the graph represents the nuclear envelope-associated heterochromatin (NE-HC), the euchromatin compartment (EC) and five concentric rings of the central heterochromatin domain that are separated by 150nm as indicated on panel B. The darkest bar positions the EC/HC interface. The labelling densities were normalized by the average labelling density of the central HC domain. (C) Same representation as in (B) for the histone marks specific for active transcription. (D) Schematic representation of a rod nucleus showing the euchromatin (white) and the heterochromatin (grey) territories. The central heterochromatin domain is separated into 5 concentric rings separated by 150 nm and analyzed independently to determine the radial variation in labelling density. The coloured dots represent schematically the distribution of the histone marks and respect the colour code used in panel (A).