ECM related gene expression levels (mRNA) of collagen type I α-2 (Col I), fibronectin (FN), fibromodulin (FMOD), hyaluronic acid synthase 3 (HAS3), hyaluronidase 1 (HYAL1) and TGF-β1 in immortalized hVFFs responding to Carbylan GSX and Matrigel at Day 7. Y-axis shows the fold of control (polystyrene or Matrigel) of each target gene (mRNA concentration ng/μl), normalized by housekeeping gene, β-actin mRNA (ng/μl). (A) Tissue culture polystyrene was used as control and mRNA level on polystyrene after seven days of culture was defined as 1.0. Y-axis demonstrates fold change of mRNA levels in 2D and 3D Carbylan GSX: Carbylan GSX; (B) Matrigel was used as control and mRNA level in Matrigel after seven days of culture was defined as 1.0. Y-axis demonstrates fold change of mRNA levels in 3D Carbylan GSX. * p<0.05, ** p<0.01.