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. Author manuscript; available in PMC: 2011 Aug 1.
Published in final edited form as: Acta Biomater. 2010 Jan 28;6(8):2940–2948. doi: 10.1016/j.actbio.2010.01.032

Table 1.

Primer Sequences and Products of RT-PCR

Gene GenBank # Forward Primer Reverse Primer Size of PCR product
COX2 NM_000963 5′-ACAGATGCAATTCCCGGACGTCTA-3′ 5′-TGGGCATGAAACTGTGGTTTGCTC-3′ 112bp
IL-6 NM_000600 5′-AAGCCAGAGCTGTGCAGATGAGTA-3′ 5′-GCTGCGCAGAATGAGATGAGTTGT-3′ 176bp
IL-8 NM_000584 5′-AGACATACTCCAAACCTTTCCACCC-3′ 5′-TCCAGACAGAGCTCTCTTCCATCA-3′ 122bp
TNF-α NM_000594 5′-AATGGTGCTCCTGGTATTGCTGGT-3′ 5′-ACCAGTGTCTCCTTTGCTGCCA-3′ 131bp
Collagen I α-2 NM_000089 5′-AACAAATAAGCCATCACGCCTGCC-3′ 5′-TGAAACAGACTGGGCCAATGTCCA-3′ 101bp
Fibronectin NM_002026 5′-ACCTACGGATGACTCGTGCTTTGA-3′ 5′-CAAAGCCTAAGCACTGGCACAACA-3′ 116bp
Fibromodulin NM_002023 5′-AACCTCAAGTACCTGCCCTTCGTT-3′ 5′-TATCACTGGTGATCTGGTTGCCGT-3′ 148bp
HAS3 NM_005329 5′-TGTGCAGTGTATTAGTGGGCCCTT-3′ 5′-TTGGAGCGCGCGGTATACTTAGTT-3′ 177bp
HYAL1 NM_000088 5′-TAACCCTGCCAGTTTCTCCATCCA-3′ 5′-AGCCAGGGTAGCATCGACATTTGA-3′ 125bp
TGF-β1 NM_000660 5′-TGCTCGCCCTGTACAACAGCA-3′ 5′-CGTTGTGGGTTTCCACCATTAGCA-3′ 126bp
β-Actin NM_001101 5′-ACGTTGCTATCCAGGCTGTGCTAT-3′ 5′-CTCGGTGAGGATCTTCATGAGGTAGT-3′ 188bp