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. 2010 Mar 30;76(5):1142–1161. doi: 10.1111/j.1365-2958.2010.07105.x

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© 2010 Blackwell Publishing Ltd

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Fig. 6 — Colocalization of Sigma A consensus sequences and verified Rex binding sites. A. Total RNA was isolated from SH1000 (w) and SH1000 Δrex (r) before and 30 min after a shift to anaerobic conditions. Equal amounts (10 µg) of total RNA were used. The transcription start sites are marked with arrows. Lanes A, C, G and T show the dideoxy sequencing ladders obtained with the equivalent primer. B. The nucleotide sequences around the respective promoter regions of Rex-regulated genes are shown. The transcriptional start points for ldh1, pflBA and adhE obtained by primer extension experiments are indicated by arrows. The potential −10 and −35 regions of SigA-dependent promoters in front of the transcriptional start points of these genes are framed.