Table 2.
Rex-regulated proteins in S. aureus.
| Ratio 30 (60) minutes after oxygen limitatione |
||||||||||
|---|---|---|---|---|---|---|---|---|---|---|
| Locus (8325)a | Proteinb | ROPc | Spotd |  |
 |
 |
 |
 |
Locus (COL)f | Description (COL)f |
| Class I proteins | ||||||||||
| SAOUHSC_00206 | Ldh1 | + | 1 | 13.4 (18.6) | 1.5 (2.8) | 1.7 (5.5) | 0.9 (0.5) | 7.7 (3.4) | SACOL0222 | l-lactate dehydrogenase |
| 2 | 16.2 (12.5) | 3.9 (2.5) | 6 (6.6) | 0.6 (0.4) | 2.7 (1.9) | SACOL0222 | ||||
| 3 | 3.5 (5.5) | 1.3 (1) | 2.2 (2.5) | 0.6 (0.4) | 1.6 (2.2) | SACOL0222 | ||||
| 4 | 8.9 (10.3) | 2 (1.4) | 3.7 (5.9) | 0.5 (0.2) | 2.4 (1.8) | SACOL0222 | ||||
| SAOUHSC_01586 | SrrA | + | 2.9 (3.1) | 1.4 (1.2) | 1 (1.6) | 1.4 (0.8) | 2.9 (2) | SACOL1535 | DNA-binding response regulator | |
| Class II proteins | ||||||||||
| SAOUHSC_00113 | AdhE | + | 1 | 13.4 (6.9) | 48.1 (12.4) | 7.9 (1.8) | 6.1 (7.1) | 1.7 (4) | SACOL0135 | Alcohol dehydrogenase, iron-containing |
| 2 | 14.6 (12.3) | 67.8 (17.8) | 8.9 (2.8) | 7.6 (6.4) | 1.6 (4.4) | SACOL0135 | ||||
| 3 | 3.9 (3.4) | 21.2 (5.3) | 2.9 (0.6) | 7.4 (9.1) | 1.4 (5.7) | SACOL0135 | ||||
| SAOUHSC_00187 | PflB | + | 1 | 1 (0.7) | 9.2 (3) | 0.5 (0.6) | 16.9 (5.5) | 1.9 (1.3) | SACOL0204 | Formate acetyltransferase |
| 3 | 1.2 (0.7) | 9.3 (3.9) | 0.3 (0.2) | 28.2 (20) | 3.6 (3.7) | SACOL0204 | ||||
| 4 | 1.2 (1.1) | 7 (3.6) | 0.1 (0.1) | 72.8 (39.6) | 12.4 (11.7) | SACOL0204 | ||||
| 5 | 0.9 (0.5) | 5.1 (2.6) | 0.1 (0.1) | 43.2 (43.1) | 7.9 (8.1) | SACOL0204 | ||||
| SAOUHSC_00608 | Adh1 | + | 2.5 (5.2) | 9.1 (3.2) | 0.8 (0.7) | 11 (4.4) | 3 (7.2) | SACOL0660 | Alcohol dehydrogenase | |
| Class III proteins | ||||||||||
| SAOUHSC_00375 | GuaA | 1 (1.6) | 2.4 (4.9) | 1.5 (1.9) | 1.6 (2.7) | 0.6 (0.9) | SACOL0461 | GMP synthase/glutamine amidotransferase protein | ||
| SAOUHSC_00535 | – | 1.3 (1.6) | 3.3 (2.2) | 1.1 (1.5) | 2.9 (1.5) | 1.2 (1.1) | SACOL0599 | Hypothetical protein | ||
| SAOUHSC_00909 | – | 0.8 (0.8) | 3.4 (1.4) | 0.5 (0.5) | 6.6 (3.1) | 1.6 (1.7) | SACOL0976 | Hydrolase, haloacid dehalogenase-like family | ||
| SAOUHSC_01064 | Pyc | 2 | 1.4 (11.4) | 1.7 (1.4) | 5.3 (6.8) | 0.3 (0.2) | 0.3 (1.7) | SACOL1123 | Pyruvate carboxylase | |
| SAOUHSC_01218 | SucD | 1.4 (1.1) | 3.8 (1.5) | 1.6 (1.2) | 2.4 (1.3) | 0.9 (0.9) | SACOL1263 | Succinyl-CoA synthetase subunit alpha | ||
| SAOUHSC_01266 | – | 1.3 (1.2) | 5.5 (4.4) | 6.5 (5.2) | 0.8 (0.8) | 0.2 (0.2) | SACOL1308 | Pyruvate ferredoxin oxidoreductase, alpha subunit | ||
| SAOUHSC_01337 | Tkt | 1.8 (1.8) | 2.6 (3.4) | 1.9 (2.3) | 1.4 (1.4) | 0.9 (0.8) | SACOL1377 | Transketolase | ||
| SAOUHSC_01605 | Gnd | 1.1 (1.5) | 5 (1.7) | 1.1 (0.8) | 4.7 (2) | 1 (1.7) | SACOL1554 | 6-phosphogluconate dehydrogenase | ||
| SAOUHSC_01737 | AspS | 1.4 (1.6) | 2 (3.4) | 2.6 (5.6) | 0.8 (0.6) | 0.5 (0.3) | SACOL1685 | Aspartyl-tRNA synthetase | ||
| SAOUHSC_01839 | TyrS | 1 (1.4) | 1.3 (3.2) | 0.8 (1.8) | 1.6 (1.8) | 1.3 (0.8) | SACOL1778 | Tyrosyl-tRNA synthetase | ||
| SAOUHSC_02142 | AldA2 | 4.5 (1.1) | 0.5 (0.8) | 1.1 (1.1) | 0.4 (0.7) | 4 (1) | SACOL1984 | Aldehyde dehydrogenase | ||
| SAOUHSC_02441 | Asp23 | 1 | 0.8 (0.6) | 3 (2.5) | 0.9 (0.8) | 3.4 (3.3) | 0.9 (0.8) | SACOL2173 | Alkaline shock protein 23 | |
| SAOUHSC_02561 | UreC | 1.9 (1.4) | 2.3 (3) | 2 (1.9) | 1.2 (1.6) | 1 (0.7) | SACOL2282 | Urease subunit alpha | ||
| SAOUHSC_02862 | ClpL | 1.5 (1.3) | 2.9 (4.1) | 0.6 (0.3) | 5.2 (12.8) | 2.6 (4) | SACOL2563 | ATP-dependent Clp protease, putative | ||
| Class IV proteins | ||||||||||
| SAOUHSC_00338 | MetE | 2 | 1.6 (1.4) | 0.3 (0.3) | 1 (1.3) | 0.3 (0.2) | 1.5 (1.1) | SACOL0428 | 5-methyltetrahydropteroyltriglutamate-homocysteine methyltransferase | |
| SAOUHSC_00365 | AhpC | 3 | 0.2 (0.8) | 0.4 (0.6) | 0.8 (1.1) | 0.6 (0.6) | 0.2 (0.8) | SACOL0452 | Alkyl hydroperoxide reductase, C subunit | |
| SAOUHSC_00794 | GapR | 0.7 (1) | 0.3 (0.6) | 0.3 (0.3) | 1 (2.2) | 2.8 (3.8) | SACOL0837 | Gap transcriptional regulator | ||
| SAOUHSC_00795 | GapA1 | 1 | 0.6 (0.7) | 0.3 (0.6) | 0.3 (0.1) | 1.1 (5.1) | 2.2 (5.7) | SACOL0838 | Glyceraldehyde 3-phosphate dehydrogenase | |
| 2 | 0.7 (1.3) | 0.2 (0.7) | 0.2 (0.2) | 1.1 (4.1) | 3.4 (7.4) | |||||
| 3 | 1 (0.6) | 0.3 (0.6) | 0.3 (0.1) | 1.1 (5.4) | 3.6 (5.3) | |||||
| SAOUHSC_00796 | Pgk | 2 | 0.8 (1) | 0.3 (0.9) | 0.2 (0.2) | 1.3 (4.7) | 3.6 (5.4) | SACOL0839 | Phosphoglycerate kinase | |
| SAOUHSC_00831 | – | 0.6 (0.6) | 0.2 (0.7) | 0.4 (0.4) | 0.6 (1.5) | 1.3 (1.2) | SACOL0872 | OsmC/Ohr family protein | ||
| SAOUHSC_00920 | FabH | 3 | 1.4 (0.2) | 0.4 (0.3) | 1.3 (0.7) | 0.3 (0.5) | 1 (0.3) | SACOL0987 | 3-oxoacyl-(acyl carrier protein) synthase III | |
| SAOUHSC_01347 | – | 0.2 (0.4) | 1.4 (0.6) | 0.5 (0.9) | 2.7 (0.6) | 0.4 (0.4) | SACOL1385 | Aconitate hydratase | ||
| SAOUHSC_01968 | – | 0.3 (0.3) | 0.3 (0.5) | 0.4 (0.3) | 0.9 (1.5) | 0.9 (0.9) | SACOL1894 | HIT family protein | ||
| SAOUHSC_02244 | – | 0.6 (0.6) | 0.3 (0.4) | 0.7 (0.4) | 0.4 (1) | 0.9 (1.4) | – | |||
| SAOUHSC_02441 | Asp23 | 3 | 0.7 (0.8) | 0.3 (0.5) | 0.6 (0.6) | 0.6 (1) | 1.2 (1.4) | SACOL2173 | Alkaline shock protein 23 | |
| SAOUHSC_02441 | Asp23 | 4 | 0.5 (0.5) | 0.2 (0.6) | 0.3 (0.3) | 0.5 (1.8) | 1.3 (1.6) | |||
a
Loci are based on TIGR annotation of S. aureus COL (http://cmr.jcvi.org).
b
Symbols are based on TIGR annotation of S. aureus COL or N315 (italic) using Geneplot of NCBI (http://www.ncbi.nlm.nih.gov).
c
The presence of a Rex binding site (ROP) in front of the respective genes indicated by +.
d
Spots representing the same protein are distinguished by an additional spot number.
e
Protein spots whose synthesis was significantly induced or repressed at least threefold in the mutant compared with the wild-type.
f
Loci and product descriptions are based on TIGR annotation of S. aureus COL using Geneplot of NCBI.