Table 3.
Strains and plasmids used in this study.
| Strains or plasmids | Description | Source or reference |
|---|---|---|
| Strains | ||
| Escherichia coli | ||
| DH5α | supE44ΔlacU169 (f80lacZDM15) hsdR17 recA1 endA1 gyrA96 thi-1 relA1 | Hanahan (1983) |
| Staphylococcus aureus | ||
| RN4220 | Restriction negative strain | Kreiswirth et al. (1983) |
| SH1000 | Functional rsbU strain derived from S. aureus 8325-4 | Horsburgh et al. (2002) |
| SH1000 Δrex (AK1) | SH1000 with a 20 bp deletion in rex | This study |
| SH1000 ΔarcR | SH1000 with a deleted arcR gene | Makhlin et al. (2007) |
| Plasmids | ||
| pJM930 | pBT2 based vector containing a I-SceI restriction site | This study |
| pAK001 | pJM930 with mutated rex gene for recombination | This study |
| pJM928 | pSPT181 shuttle vector, constitutively expressing I-SceI | Heinrichs et al. (1996) |