Table 1.
Bacterial Strains and plasmids used in this study.
| Strain or plasmid | Relevant genotype/phenotype | Reference or source |
|---|---|---|
| E. coli strains | ||
| W3110 | Wild type for chemotaxis; F- λ- IN(rrnD-rrnE)1 rph-1 | Laboratory stock |
| RL001 | W3110 ΔcobB::Km | This study |
| RL002 | W3110 Δacs::Km | This study |
| RL0012 | W3110 ΔcobBΔacs::Km | This study |
| RL003 | W3110 ΔcheAΔcheZ::Km | This study |
| RL0031 | W3110 ΔcheAΔcobBΔcheZ::Km | This study |
| RL0032 | W3110 ΔcheAΔacsΔcheZ::Km | This study |
| AJW613 | Wild type for chemotaxis; ΔlacX74 thi-1 thr-1(Am) leuB6 metF159(Am) rpsL136λlacY | A.J. Wolfe |
| AJW803 | AJW613 Δacs::Km-1 | Kumari et al. (1995) |
| DH5α | Host for plasmid propagation | Laboratory stock |
| BL21(DE3) | Host for protein expression | Laboratory stock |
| AD494(DE3) | Host for protein expression, KmR | Laboratory stock |
| Plasmids | ||
| pKD46 | Red recombinase expression plasmids, ApR | Yale CGSC |
| pKD4 | Template plasmids containing a kanamycin resistance gene flanked by FRT sites, ApR, KmR | Yale CGSC |
| pCP20 | FLP helper plasmid, ApR, CmR | Yale CGSC |
| pET20b-cheY | CheY-His expression plasmids, ApR | This study |
| pTricHis2C-acs | Acs-His expression plasmids, ApR | This study |
| pET32a-cobB | His-CobB expression plasmids, ApR | This study |
| pET28a-fliM | His-FliM expression plasmids, KmR | This study |