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. 2010 Apr 1;76(5):1175–1190. doi: 10.1111/j.1365-2958.2010.07130.x

Fig. 3.

Fig. 3

P46 Overexpressing Parasites in the Mouse Infection Model. A. L. major hsp100-/- (solid triangles) or L. major hsp100-/-[pcosP46] (open triangles) were inoculated into footpads of BALB/c mice (n = 5). Footpad swelling [mm] was monitored at weekly intervals. B. Infection of BALB/c mice (n = 4) with L. major (solid squares) or L. major[pcosP46] (open squares). Footpad swelling was monitored weekly. C. Real-time RT-PCR. The relative P46-specific mRNA levels in log phase promastigotes of L. major hsp100-/-, L. major hsp100-/-[pcosp46], L. major wild type and L. major[pcosP46] were determined by whole cell RNA extraction, cDNA synthesis and subsequent P46-specific real-time PCR. Results are given as multiples of L. major wild type expression levels. D. C57BL/6 mice (n = 4) were infected with either L. major (solid squares) or L. major[pcosP46] (open squares). Footpad swelling was monitored over 110 days.