Table 2.

Differentially expressed proteins in sera of normal subjects and asymptomatic WD patients, identified using MALDI-TOF MS and MS/MS

Spot no.	Protein name	Up/down-regulated female/malea)	Accession no.		MALDI-TOF MS			MALDI-TOF MS/MSb)
			NCBInr	Swiss-Prot	Score	Decision DB (MASCOTc)/ProFoundd))	Matched peptides (%)	MASCOT score	Matched peptides (%)
1	Complement factor B, preproprotein	−5.5/−3.1	gi∣4502397	Q53F89	103	MASCOT	11/25 (44%)	242	4/43 (9%)
2	Alpha-2-macroglobulin precursor	−4.3/−2.7	gi∣112911	P01023	1531.3e-03	MASCOTProFound	17/25 (68%)	469	4/53 (8%)
3	Complement factor B preproprotein	−2.4/−2.2	gi∣14124934	Q53F89	82	MASCOT	25/210 (12%)	141	3/76 (4%)
4	Hemoglobin Gower 2 epsilon	–/+3.4	gi∣223114	P02100	71	MASCOT	6/40 (15%)
5	Nesprin-1 beta 2	+4.4/–	gi∣370178	Q8NF91	65	MASCOT	42/135 (31%)
6	Immunoglobulin heavy chain variable region	+3.0/–	gi∣18307244	P01764	34	MASCOT	5/128 (4%)
7	Ig heavy chain V region (clone P1–53)	+3.3/–	gi∣484705	P23083	66	MASCOT	5/25 (20%)
8	Isocitrate dehydrogenase 3 (NAD+) alpha	−2.1/−2.8	gi∣5031777	P50213				15	1/49 (2%)
9	Immunoglobulin heavy chain variable region	–/+2.5	gi∣112701283	P01764	60	MASCOT	5/42 (12%)
10	Cytokeratin 8	−2.0/–	gi∣30313	P05787				63	1/47 (2%)
11	Complement component C3 precursor	−2.3/−2.1	gi∣4557385	P01024	119	MASCOT	17/115 (15%)	302	5/72 (7%)
12	Thiol-specific antioxidant protein (PRX2)	–/−2.0	gi∣438069	P32119	87	MASCOT	13/155 (8%)	320	6/71 (9%)
13	Smad ubiquitination regulatory factor 1	−6.9/–	gi∣10047327	Q9HCE7				24	1/44 (2%)
14	Haptoglobin precursor	−3.8/–	gi∣67586	P00738				24	1/25 (4%)

^a)Fold changes in values obtained from image analysis of spots between control subjects and patients.

^b)Individual ions scores >35 indicate identity or extensive homology (p<0.05). Ions score is –10 log(p), where p is the probability that the observed match is a random event.

^c)Protein scores greater than 64 are significant (p<0.05) for MS data.

^d)Expectation value scoring was used, which indicates the quality or significance of the match.