Fig. 4.

Comparison of the proportion of 2B4-expressing cells in gated populations from peripheral blood mononuclear cells (PBMCs) between systemic lupus erythematosus (SLE) patients (n = 45) and healthy controls (HC) (n = 30). (a) Lymphocyte and monocyte populations were gated based on forward- and side-scatter. (b) T cells stained with anti-CD3-fluorescein isothiocyanate (FITC) were gated. (c) Natural killer (NK) cells stained with anti-CD56-allophycocyanin (APC) were gated. (d) Monocytes stained with anti-CD14-FITC were gated. For each gate, the proportion of 2B4-expressing cells was calculated by subtracting the percentage of phycoerythrin (PE)-conjugated mouse IgG1-stained cells from the percentage of PE-conjugated anti-2B4 antibody-stained cells. Asterisk indicates significant differences between HC and SLE groups.