Fig. 3.

Immunohistochemical findings of phosphorylated Smad3 (p-Smad3) in lungs of B6 mice treated with SB-431542. (a) Lungs were harvested from B6 mice at 6 h after treatment with interleukin (IL)-18/IL-2 with or without SB-431542 for 3 days. The tissues were stained immunohistochemically with anti-pSmad3 antibody. Arrow: pSmad3-positive cells. Original magnification: ×200. (b) In lungs of mice treated with IL-18/IL-2 with or without SB-431542, the percentage of p-Smad3-positive cells per total cells was calculated in five fields under ×200 magnification. Data are mean ± standard error of the mean of three mice per group. *P < 0·05; Student's t-test.