Fig. 4.

Flow cytometric analysis of pulmonary lymphocytes in B6 mice treated with SB-431542. (a) Pulmonary lymphocytes were harvested from B6 mice at 24 h after treatment with interleukin (IL)-18/IL-2 with or without SB-431542 for 3 days, as described in Materials and methods. Pulmonary lymphocytes were stained with phycoerythrin (PE)-conjugated anti-natural killer (NK)1·1 and PE/cyanine 7 (Cy7)-conjugated anti-CD3ε monoclonal antibodies. (b) Left: proportion of NK cells relative to pulmonary lymphocytes in mice treated with IL-18/IL-2 and with or without SB-431542. Right: proportion of NK cells relative to splenocytes in B6 mice treated with IL-18/IL-2 with or without SB-431542. Data are mean ± standard error of the mean of three mice per group. *P < 0·05; Student's t-test.