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. 2010 Jun;160(3):394–402. doi: 10.1111/j.1365-2249.2010.04094.x

Fig. 5.

Fig. 5

Histological and flow cytometric analysis of pulmonary lymphocytes in Smad3–/– mice. (a) Lungs were harvested from B6 and Smad3–/– mice at 24 h after treated with interleukin (IL)-18/IL-2 for 3 days. Lung tissues were stained with haematoxylin and eosin. Original magnification: ×100. (b) Pulmonary lymphocytes were harvested from B6 and Smad3–/– mice at 24 h after treatment with IL-18/IL-2 for 3 days, as described in Materials and methods. Pulmonary lymphocytes were stained with phycoerythin (PE)-conjugated anti-natural killer (NK)1·1 and PE/cyanine 7 (Cy7)-conjugated anti-CD3ε monoclonal antibodies. (c) Proportion of NK cells relative to pulmonary lymphocytes in B6 and Smad3–/– mice treated with IL-18/IL-2. Data are mean ± standard error of the mean of three mice per group. *P < 0·05; Student's t-test.