Fig. 1.

CCR6 characterizes lymphoid tissue inducer (Lti) cells within cryptopatches. Fluorescence activated cell sorter (FACS) analysis of lin^- c-kit⁺ lamina propria cells (a) revealed a significant proportion of CCR6-expressing cells within the lin^- c-kit⁺ cell fraction [approximately 15–20%; analysis of heterozygous enhanced green fluorescent protein (EGFP)–CCR6 knock-in mice, n = 10]. However, when analysed by immunohistochemistry (b), a significantly higher number of cryptopatch cells expressed this receptor (75 ± 8%, n = 10, P < 0001). All control stainings were negative.