Table 1. Cloning efficiencies and radiosensitivity parametersa of in vitro irradiated tumor cell lines untreated and pretreated with the Hsp90 inhibitors.
| Cell line | Plating efficiency | SF2 | α (Gy−1) | β (Gy−2) | D10 (Gy)b | Factor (D10 control)/(D10+inh.) |
|---|---|---|---|---|---|---|
| A549 – contr. | 0.8±0.1 | 0.70±0.05 | 0.1±0.03 | 0.04±0.01 | 6 | 1.0 |
| +AUY922 | 0.5±0.01 | 0.33±0.06 | 0.4±0.2 | 0.04±0.05 | 4 | 1.5 |
| +BEP800 | 0.8±0.1 | 0.52±0.1 | 0.2±0.1 | 0.04±0.01 | 5 | 1.2 |
| +17DMAG | 0.7±0.1 | 0.41±0.08 | 0.3±0.2 | 0.07±0.05 | 4 | 1.5 |
| GaMG – contr. | 0.3±0.1 | 0.84±0.01 | 0.01±0.01 | 0.04±0 | 8 | 1.0 |
| +AUY922 | 0.1±0 | 0.46±0.04 | 0.1±0.07 | 0.1±0 | 4 | 1.8 |
| +BEP800 | 0.3±0.1 | 0.57±0.08 | 0.1±0.1 | 0.07±0.01 | 5 | 1.4 |
| +17DMAG | 0.1±0.05 | 0.44±0.02 | 0.1±0 | 0.1±0 | 4 | 1.8 |
| HT1080 – contr. | 0.1±0.04 | 0.81±0 | — | 0.05±0 | 7 | 1.0 |
| +AUY922 | 0.02±0.03 | 0.63±0.2 | 0.1±0.2 | 0.06±0.01 | 5 | 1.4 |
| +BEP800 | 0.1±0.03 | 0.59±0.2 | 0.06±0.08 | 0.08±0.03 | 5 | 1.4 |
| +17DMAG | 0.03±0.01 | 0.70±0.1 | — | 0.1±0.02 | 5 | 1.4 |
| SNB19 – contr. | 0.3±0.1 | 0.53±0.3 | 0.3±0.4 | 0.03±0.04 | 6 | 1.0 |
| +AUY922 | 0.2±0.1 | 0.40±0.3 | 0.4±0.4 | 0.07±0.04 | 5 | 1.3 |
| +BEP800 | 0.2±0.1 | 0.45±0.2 | 0.3±0.2 | 0.06±0.01 | 5 | 1.3 |
| +17DMAG | 0.1±0.1 | 0.53±0.2 | 0.1±0.1 | 0.1±0 | 4 | 1.3 |
Abbreviations: contr.=control; inh.=inhibitor.
a
Mean (±s.d.) from at least two independent experiments.
b
D10 is the radiation dose required to reduce clonogenic survival by 10%.