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. 2010 May 11;102(11):1618–1626. doi: 10.1038/sj.bjc.6605689

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Copyright © 2010 Cancer Research UK

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In vivo assay of CSQT-2 cell line. (A) Subcutaneous growth of CSQT-2 tumour. 100 000, 10 000 or 1000 cells of CSQT-2 were inoculated at the flanks of nude mice. Three mice were used in each group with different cell numbers. (B) Orthotropic growth of CSQT-2 tumour labeled with luciferase. CSQT-2 cells labeled with luciferase were first injected into the flank of nude mice. After subcutaneous tumour appeared, it was harvested and inoculated into the liver of fresh nude mice. The orthotropic tumour growth was measured by luciferase activity detection by IVIS system. (C) Metastatic growth of CSQT-2 tumour via cardiac injection. 500 000 cells of CSQT-2 were injected into the left ventricle of nude mice for metastasis. Shown was the metastasis 4 weeks post injection. (D) Metastatic growth of CSQT-2 tumour via intra-spleen injection. 500 000 cells of CSQT-2 were injected into the spleen of nude mice for metastasis. Shown was the metastasis 4 weeks post injection. (E) PVTT formed by CSQT-2 orthotropic tumour. The mouse livers in (B) were harvested for fixation and H&E staining to detect whether PVTT was formed. Three typical PVTT sections were shown.