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. Author manuscript; available in PMC: 2010 Jun 10.
Published in final edited form as: Methods Mol Biol. 2008;475:263–274. doi: 10.1007/978-1-59745-250-2_15

Table 1. Fluorescent proteins useful for live imaging of myoblast fusion.

EGFP=enhanced green fluorescent protein; EYFP=enhanced yellow fluorescent protein; NLS=nuclear localization signal; DsRed=Discosoma Red; H2B=human histone 2B, mCD8 = mouse CD8. Excitation and emission represent approximate maximum values.

Protein Localization Excitation/Emission Reference/Source
EGFP cytoplasm 488nm/509nm Clontech, (24, 25)
EYFP cytoplasm 514nm/527nm Invitrogen, (24)
GFP::actin actin
cytoskeleton		 488nm/509nm (16)
GFP::moesin actin
cytoskeleton		 488nm/509nm (26)
GFP::α-Tubulin Microtubules 488nm/509nm (18)
NLS::EGFP Nucleus 488nm/509nm (25)
NLS::DsRed.T4 nucleus 556nm/586nm (15)
H2B:YFP nucleus 514nm/527nm (27)
Src::GFP cell membrane 488nm/509nm (9)
mCD8::GFP cell membrane 488nm/509nm (17)