Figure 3.

(A) Representative flow cytometry histogram of cellular green fluorescence displaying a control sample (solid curve) and samples subjected to low (dashed curve) and high (dotted curve) shear environments, which display an increase in green fluorescence indicating intracellular uptake of calcein. The samples were subjected to shear by flow of the cell suspension at 100 ml/h though channels of 100 μm in length and diameters of 60 μm for the low shear condition and 50 μm for the high shear condition (B,C) Representative fluorescent micrographs of cells after exposure to shear in microchannel flow (at the high shear condition described above), which depicts (B) viable cells with intracellular uptake of green-fluorescent calcein and (C) non-viable cells stained with red-fluorescent propidium iodide. The same view of the same cell sample is shown in parts (B) and (C) using different optics. Stained cells appear as black circles and unstained cells appear as bright circles.