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. Author manuscript; available in PMC: 2011 May 1.
Published in final edited form as: Mech Ageing Dev. 2010 Apr 2;131(5):330–337. doi: 10.1016/j.mad.2010.03.009

Fig.4.

Fig.4

Age-dependent changes in the level of antioxidant enzymes in mouse tissue. Total cell extracts of 4-, 10-, and 20-month-old mouse liver (A), kidney (B), heart (C), pectorals (D), and quadriceps (E) were immunoblotted against the antioxidant enzymes MnSOD, Cu/ZnSOD, and catalase; GAPDH was used as the control (upper panels). Lower panels show the quantified level of antioxidant enzymes normalized to GAPDH, with each young tissue set at 1, except for MnSOD where mitochondrial volume was used. Extracts from 6–7 animals were used. The level of MnSOD was normalized to activity of citrate synthase. Error bars represent standard errors, and asterisks denote p<0.05 when compared to young (4-month old) animals.