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. 2010 Apr 13;19(13):2630–2637. doi: 10.1093/hmg/ddq150

Figure 1.

Figure 1.

Analysis of the allelic origin of de novo t(11;22) translocations. (A) Nested PCR strategy for determining allelic origin of the der(22) of the t(11;22). PATRRs are shown by thick arrows, and the AT-rich region flanking the PATRR22 is indicated by a hatched box. PCR primers are shown by thin arrows to indicate the location and orientation. (B) Representative results of the nested PCR. For an A/C heterozygote, the first PCR can distinguish the allelic origin of the PCR products based on product size (upper panel). For a B/C heterozygote, the size of the first PCR product is similar to one another (middle panel). The second PCR can clearly distinguish between the allelic origins (lower panel). Two bands were observed in lane 2, suggesting that the template DNA includes two or more translocations. M, size markers; P, DNA from t(11;22) translocation carrier served as positive controls.