Fig. 2.

Farnesol directly applied to titanium alloy discs inhibits biofilm formation by Staphylococcus aureus. In the “wet farnesol on titanium disc” group, the discs were incubated in 30-mM farnesol and transferred to tissue-culture plates, and the bacterial suspension was added immediately. In the “dried farnesol on titanium disc” group, the discs were incubated in 30-mM farnesol and then air-dried prior to transfer to the tissue-culture plates. The number of viable bacteria was quantified after three hours of incubation. The effect of farnesol was evaluated with fluorescence microscopy with both low (1.6×) and high (8×) magnification (A), and the number of viable bacteria was quantified (B). CFU = colony-forming units.