Fig. 7.

The effects of farnesol on MC3T3-E1 pre-osteoblastic cells are not reversed by subsequent culture in the absence of farnesol. MC3T3-E1 cells were incubated on titanium alloy discs in the presence or absence of 30-mM farnesol for two hours (A). Following the incubation, the cells were washed twice with Dulbecco phosphate-buffered saline solution and further incubated in cell culture media without farnesol for two or five days (B). The cellular morphology was assessed with fluorescence microscopy following staining with Texas Red-X phalloidin and DAPI (magnification, 20×).