FIG. 1.

DNA-binding activity of NF-κB family members. We used 5 μg of nuclear extracts from mock, P2, and P18 PICV-infected cells to assay DNA binding of NF-κB p65 and p50 to the κB consensus site in vitro. (A) The bar graphs show the results from three independent infections. Error bars show the standard error of the mean. Data are normalized to the DNA-binding activity of a triplicate set of nuclear extracts from time-point zero. Asterisks indicate significant differences (p <0.05 by Students T-test). (B) Line graph representations are used to illustrate the oscillatory nature of the responses. The y axis shows absorbance, and the x axis is hours post-infection.