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. 2009 Dec;11(4):523–533. doi: 10.1089/clo.2009.0020

FIG. 4.

FIG. 4.

Embryoid body formation upon primary and secondary differentiation is significantly enhanced in Twist-2 silenced ES cells. (A) Results from primary embryoid body (EB) colony counting assay. The stable LV-siTwist-2 ES cells generates a significantly increased frequency of primary embryoid bodies with the size of larger than 100 μm (n = 8 per group, 60 × 15 mm plates). Data are shown as means ± standard error mean (SEM) of two independent experiments. *p < 0.05, two-tail t-test; LV-siTwist-2 versus LV-siGFP ES cells. (B) Representative images for different colonies, colony forming unit (CFU)-erythroid (E), CFU-GM (granulocyte-macrophage), burst forming unit (BFU)-E, CFU-M (macrophage), and secondary EB (2nd EB) at day 14 of secondary hematopoietic differentiation derived from LV-siTwist-2 ES cells. (C) Results of total colonies counts at day 14 of secondary hematopoietic differentiation. The stable LV-siTwist-2 ES cell generated a significantly increased frequency of secondary hematopoietic colonies (n = 8 per group, 60 × 15 mm plates). Data are shown as means ± standard error mean (SEM) of two independent experiments. *p < 0.05, two-tail t-test; LV-siTwist-2 versus LV-siGFP ES cells.