Skip to main content
. 2009 Dec;11(4):523–533. doi: 10.1089/clo.2009.0020

FIG. 6.

FIG. 6.

Enhanced generation of myeloid cells differentiated from Twist-2 silenced ES cells in response to stimulation with TLR agonists. (A) Enhanced primary EB formation of Twist-2-silenced ES cells by LPS stimulation. Primary embryoid body (EB) formation assays were performed by counting EB (n = 6 per group, 60 × 15-mm plates) after 10 days culture with, or without LPS (10 μg/mL) stimulation. NS, no stimulation. Data are shown as means ± standard error mean (SEM) of threee independent experiments. *p < 0.05, two-tail t-test; LV-siTwist-2 versus LV-siGFP. (B) Enhanced myeloid differentiation of Twist-2-silenced ES cells by LPS stimulation. Increased Gr-1+ or F4/80+ myeloid cells differentiated from LV-siTwist-2 ES cells after 14-day culture in the presence of LPS (10 μg/mL) stimulation, or without stimulation. Data presented as means ± standard error mean (SEM) of three independent experiments. *p < 0.05, two-tail t-test; LV-siGFP versus LV-siTwist-2; **p < 0.05, LPS versus NS.