Table 1.
I-Ab and I-As Restricted Epitopes
| I-Ab restricted epitopes | Spots per 106 T cells | ± SE (n) | I-As restricted epitopes | Spots per 106 T cells | ± SE (n) |
|---|---|---|---|---|---|
| HA 16: 90 KESWSYIVETPNPENGT 106 | 100 | 23 (13) | HA 21: 120 EQLSSVSSFERFEIFPK 136 | 306 | 53 (10) |
| HA 35: 203 NQRALYHTENAYVSVVS 219 | 119 | 14 (25) | HA 22: 126 SSFERFEIFPKESSWPN 142 | 663 | 66 (16) |
| HA 52: 304 SSLPFQNVHPVTIGECP 320 | 93 | 29 (5) | HA 23: 132 EIFPKESSWPNHTVTGV 148 | 300 | 48 (10) |
| HA 53: 310 NVHPVTIGECPKYVRSA 326 | 56 | 21 (3) | HA 24: 138 SSWPNHTVTGVSASCSH 154 | 91 | 19 (10) |
| NA 19: 108 IGSKGDVFVIREPFIS 123 | 34 | 5 (5) | HA 25: 144 TVTGVSASCSHNGKSSF 160 | 426 | 48 (14) |
| NA 20: 113 DVFVIREPFISCSHLE 128 | 134 | 19 (17) | HA 28: 162 RNLLWLTGKNGLYPNLS 178 | 263 | 40 (10) |
| NA 30: 171 NSKFESVAWSASACHDG 187 | 168 | 31 (10) | HA 38: 221 HYSRRFTPEIAKRPKVR 237 | 84 | 13 (10) |
| NA 31: 177 VAWSASACHDGMGWLTI 193 | 195 | 46 (10) | HA 53: 310 NVHPVTIGECPKYVRSA 326 | 123 | 25 (10) |
| NA 47: 273 NFHYEECSCYPDTGTVM 289 | 174 | 25 (19) | HA 54: 316 IGECPKYVRSAKLRMVT 332 | 191 | 46 (10) |
| NA 56: 327 RPKDGEGSCNPVTVDGA 343 | 86 | 33 (4) | HA 55: 322 YVRSAKLRMVTGLRNIP 338 | 93 | 20 (10) |
| NA 76: 446 FCGVNSDTANWSWPDGA 462 | 61 | 12 (3) | HA 57: 334 LRNIPSIQSRGLFGAIA 350 | 243 | 40 (10) |
| NA 77: 452 DTANWSWPDGAELPFTI 468 | 170 | 30 (13) | HA 65: 380 DQKSTQNAINGITNKVN 396 | 61 | 8 (10) |
| NP 25: 145 DTTYQRTRALVRTGMDP 161 | 56 | 18 (2) | HA 66: 386 NAINGITNKVNSVIEKM 402 | 428 | 60 (14) |
| NP 44: 258 FLARSALILRGSVAHKS 274 | 381 | 41 (10) | NA 45: 261 GKVTKSIELNAPNFHYE 277 | 161 | 23 (15) |
| NP 45: 264 LILRGSVAHKSCLPACV 280 | 418 | 54 (10) | NA 47: 273 NFHYEECSCYPDTGTVM 289 | 272 | 54 (4) |
| NP 46: 270 VAHKSCLPACVYGPAVA 286 | 33 | 13 (5) | NA 49: 285 TGTVMCVCRDNWHGSNR 301 | 55 | 35 (4) |
| NP 47: 276 LPACVYGPAVASGYDFE 292 | 157 | 15 (20) | NA 61: 357 VWIGRTKSNRLRKGFEM 373 | 112 | 19 (9) |
| NP 48: 282 GPAVASGYDFEKEGYSL 298 | 53 | 17 (7) | NP 4: 19 RQNATEIRASVGRMIGG 35 | 57 | 25 (6) |
| NP 52: 306 LLQTSQVYSLIRPNENP 322 | 848 | 106 (16) | NP 16: 91 KTGGPIYKRVDGKWVRE 107 | 230 | 45 (10) |
| NP 53: 312 VYSLIRPNENPAHKSQL 328 | 438 | 66 (10) | NP 17: 97 YKRVDGKWVRELVLYDK 113 | 403 | 61 (14) |
| NP 71: 420 FDKTTIMAAFTGNTEGR 436 | 39 | 16 (4) | NP 45: 264 LILRGSVAHKSCLPACV 280 | 208 | 49 (4) |
| M1 2: 7 VETYVLSIVPSGPLKAE 23 | 66 | 11 (7) | NP 46: 270 VAHKSCLPACVYGPAVA 286 | 1139 | 130 (17) |
| M1 3: 13 SIVPSGPLKAEIAQRLE 29 | 65 | 12 (12) | NP 47: 276 LPACVYGPAVASGYDFE 292 | 33 | 9 (8) |
| M1 24: 139 TTESAFGLICATCEQIA 155 | 55 | 20 (6) | NP 55: 324 HKSQLVWMACNSAAFED 340 | 38 | 8 (8) |
| NS1 13: 72 ESDEAFKMTMASALASR 88 | 71 | 17 (12) | NP 58: 342 RVSSFIRGTRVLPRGKL 358 | 220 | 41 (10) |
| NS1 14: 78 KMTMASALASRYLTDMT 94 | 63 | 12 (13) | NP 74: 438 SDMRAEIIKMMESARPE 454 | 217 | 37 (10) |
| NS1 19: 108 KQKVAGPLCVRMDQAIM 124 | 91 | 14 (21) | NP 75: 444 IIKMMESARPEEVSFQG 460 | 224 | 31 (14) |
| NS1 31: 180 VLIGGLEWNDNTVRVSE 196 | 62 | 9 (14) | M1 18: 103 LKREITFHGAKEIALSY 119 | 63 | 11 (10) |
| M1 31: 181 LASTTAKAMEQMAGSSE 197 | 208 | 19 (10) | |||
| M1 36: 210 RQMVQAMRAIGTHPSSS 226 | 157 | 18 (7) | |||
| NS1 10: 54 IETATCVGKQIVERILK 70 | 144 | 16 (2) |
Summary of CD4 peptide epitopes identified in C57BL/10 mice (left) and in SJL mice (right) in response to primary influenza infection. Indicated for each peptide epitope are the average spot count per million CD4 T cells, the standard error (SE), and the number of experiments in which the peptide was tested (n). Major epitopes are indicated in bold. Underlined residues represent the core epitope as determined by use of truncated peptides (not shown).