Table 1. Primers and Probes used for Real-Time PCR Analysis.
Target | Primers and Probes | Sequence (5′-3′) |
IFN-β (NM_010510) | Forward | CGGACTTCAAGATCCCTATGGA |
Reverse | TGGCAAAGGCAGTGTAACTCTTC | |
Probe | ATGACGGAGAAGATGC | |
IL-12 p40 (NM_008352) | Forward | TGGAGCACTCCCCATTCCT |
Reverse | TGCGCTGGATTCGAACAA | |
Probe | CTTCTCCCTCAAGTTC | |
IL-10 (NM_010548) | Forward | GATGCCCCAGGCAGAGAA |
Reverse | CACCCAGGGAATTCAAATGC | |
Probe | CATGGCCCAGAAAT | |
Beta actin (NM_007393) | Forward | CGATGCCCTGAGGCTCTTT |
Reverse | TGGATGCCACAGGATTCCA | |
Probe | CCAGCCTTCCTTCTT |