Figure 1. Peptide fragments of BoNT/B light and heavy chains.
Diagram is drawn to scale to facilitate size and location comparison between peptide fragments. The transmembrane (A442 to S858) and receptor-binding (E859 to E1291) domains are indicated [27]. Peptide fragments were expressed as fusions to GST at the N-terminal. N- and C-terminal amino acids of each peptide fragment are indicated. Fragments used as antigens to generate monoclonal antibodies in mice are indicated with an asterisk.