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. 2010 Jun 10;5(6):e11047. doi: 10.1371/journal.pone.0011047

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This is an open-access article distributed under the terms of the Creative Commons Public Domain declaration which stipulates that, once placed in the public domain, this work may be freely reproduced, distributed, transmitted, modified, built upon, or otherwise used by anyone for any lawful purpose.

This is an open-access article distributed under the terms of the Creative Commons Public Domain declaration, which stipulates that, once placed in the public domain, this work may be freely reproduced, distributed, transmitted, modified, built upon, or otherwise used by anyone for any lawful purpose.

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BoNT/B was separated on 10% SDS-PAGE in the presence (+) and absence (−) of 10 mM DTT, transferred to nitrocellulose membranes and probed with mAbs F24-1, F26-16, F27-33, F29-40 and MCS6-27. Bands showing the intact holotoxin (Holo.), heavy chain (Hc) and light chain (Lc) are indicated. Protein relative molecular weight (rMW) is indicated in kDa.