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. 2010 Jun 10;5(6):e11047. doi: 10.1371/journal.pone.0011047

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This is an open-access article distributed under the terms of the Creative Commons Public Domain declaration which stipulates that, once placed in the public domain, this work may be freely reproduced, distributed, transmitted, modified, built upon, or otherwise used by anyone for any lawful purpose.

This is an open-access article distributed under the terms of the Creative Commons Public Domain declaration, which stipulates that, once placed in the public domain, this work may be freely reproduced, distributed, transmitted, modified, built upon, or otherwise used by anyone for any lawful purpose.

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MAb MCS6-27 was used as capture antibody, rabbit anti-BoNT/B polyclonal (Metabiologics) was used in conjunction with an HRP-conjugated, goat anti-rabbit polyclonal antibody as detector. Data are presented ±1 standard error, n = 3. Horizontal dashed lines represent the limit of detection (L.O.D., zero toxin plus 3 standard deviations) and the limit of quantitation (L.O.Q., zero toxin plus 5 standard deviations).