Table 1. Primers.

Primer	Sequence	Constructs
B-LcF	GGATCCATGCCAGTTACAATAAATAATTTTAATTATAATG	Lc, L1
B-LcR	CTCGAG TTATTTAACACTTTTACACATTTGTATCTTATATAC	Lc, L2
B-LcintF	GGATCCGCAAGTATATTTAATAGACG	L2
B-LcintR	CTCGAG TTAGCCTTTGTTTTCTTGAAC	L1
B-HcF	GGATCCGCTCCAGGAATATGTATTGATGTTG	Hc,H1,H4
B-HcR	CTCGAG TTATTCAGTCCACCCTTCATCTTTAG	Hc, H3, H5
B-HcintR1	CTCGAG TTAGCTATTATATTTATTAAACATTTC	H1
B-HcintF2	GGATCCGAAATTTTAAATAATATTATCTTAAATTTAAG	H2, H5
B-HcintR2	CTCGAG TTAGCTATATGATTGAATTTTATATC	H2, H4
B-HcintF3	GGATCCGAATATTTAAAAGATTTTTGGGG	H3
M13F	GTAAAACGACGGCCAG	seq. pCR4 plsamids
M13R	CAGGAAACAGCTATGAC	seq. pCR4 plasmids
B-intseqF	CAATAGATAATGCTTTAACTAAAAGAAATG	seq. Hc,H1
B-intseqR	GTGTTCTATCTATATCACCATC	seq. Hc
pGS-F	CAAATTGATAAGTACTTGAAATCC	seq. pGS-21a plasmids
pGS-R	GCTAGTTATTGCTCAGAGG	seq. pGS-21a plasmids

Sites for restriction enzymes BamHI (GGATCC), XhoI (CTCGAG), are shown underlined. Stop codons are shown in bold, in the 3′ to 5′ (TTA) orientation. The third column indicates which peptide fragments each primer was used to construct (See Figure 1). Primers used only for sequencing are indicated by the abbreviation “seq.”