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. 2010 Jun 10;5(6):e11045. doi: 10.1371/journal.pone.0011045

Figure 2. Quercetin exerts a concentration-dependent effect on the formation of the CSPα dimer in rat cortical neurons and CAD cells.

Figure 2

(A) Western blot of cultured rat cortical neurons were treated with indicated concentrations of quercetin for 24 hours prior to lysis. Equal amounts of cellular protein were resolved by SDS-PAGE as confirmed by ponceau S staining. (B) CAD cells were transiently transfected with 1.0 µg c-myc-CSPα DNA and treated with indicated concentrations of quercetin for 24 hours prior to lysis. Following separation of cellular protein (30 µg) by SDS-PAGE, CSPα, Hsc70, Hsp40, Rdj2, syntaxin and Gαs were detected by Western analysis. β-actin is shown as a loading control. Data are representative of three separate experiments. (C) Native CSPα was detected in adult rat brain by Western analysis with a monoclonal anti-CSPα antibody. Twenty-five micrograms of unfractionated tissue homogenate isolated from the indicated regions of rat brain were separated by SDS-PAGE, transferred to PVDF and probed. Arrows indicate the CSPα dimer at ∼72 kDa; * indicates a palmitoylated CSPα monomer at ∼34 kDa. Actin is shown as a loading control.