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. 2010 Jun 10;5(6):e11045. doi: 10.1371/journal.pone.0011045

Figure 9. Epigallocatechin gallate (EGCG) stimulates formation of CSPα-CSPα dimers.

Figure 9

(A) CAD cells were transiently transfected with 1.0 µg c-myc-CSPα DNA and treated with indicated concentrations of EGCG for 24 hours prior to lysis. Following separation of cellular protein (30 µg) by SDS-PAGE, CSPα, was detected by Western analysis. β-actin is shown as a loading control. Arrow indicates CSPα dimer at ∼72 kDa. (B) Quantification of the CSPα dimer to monomeric palmitoylated CSPα ratio under control, 100 µM quercetin and 200 µm EGCG conditions. Numbers in parentheses indicate the numbers of experiments; error bars denote standard errors.