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. 2010 Jun 10;5(6):e11041. doi: 10.1371/journal.pone.0011041

Figure 3. Drug susceptibility assay of mutant variants of CaMDR1-GFP.

Figure 3

A: Drug resistance profile of wild type and mutant CaMDR1-GFP yeast strains as determined by spot assay. 5 µl of five-fold serial dilutions, namely 1 (1∶5), 2 (1∶25), 3 (1∶125) and 4 (1∶625), of each strain was spotted on to YEPD plates as described previously in the absence (control) and presence of the following drugs: FLU (0.20 µg/ml), MTX (65 µg/ml), CER (3 µg/ml), ANISO (3 µg/ml), CYH (0.20 µg/ml) and 4-NQO (0.20 µg/ml). Growth differences were recorded following incubation of the plates for 48 hrs at 30°C. Growth was not affected by the presence of the solvents used for the drugs (data not shown).