Figure 7. Comparative plot of Conservation, RE_NULL and CRE_S across the entire alignment.

The three scores: Conservation in DHA1 family (black), RE_NULL across DHA1 family (red) and CRE_S (green) are drawn as a line graph for all the alignment positions in the bottom panel of the Figure. Locations of the TMS are marked by black bars on the x-axis. These twelve TMS are shown separately as an inset in the top panel so to highlight the effectiveness of CRE_S over RE and traditional conservation. The conservation scores remain almost constant throughout the TMS while RE_NULL is more prominent in the N-terminal half of the protein. High CRE_S positions span all the TMS, thus showing the functionally relevant residues to be scattered over both the halves of the protein.