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. 2010 Jun 10;6(6):e1000951. doi: 10.1371/journal.ppat.1000951

Figure 6. EBNA3C-mediated regulation of p16INK4A does not require Rb.

Figure 6

(A) Western blot analysis of whole cell lysates from two LCL 3CHT (-D and -E) cultured with (+) or without (-) HT for 26 days. Although Rb is undetectable in LCL 3CHT-E, when HT is removed from the growth medium, EBNA3C decreases and p16INK4A (p16) increases. E2F1 and γ-tubulin (γ-tub) levels do not alter. (B) Steady-state levels of Rb mRNA in LCL 3CHT-E relative to 3 other LCL 3CHT and a WT-BAC LCL all cultured with HT, quantified by qRT-PCR. (C) ChIP analysis of H3K27Me3 distribution across the p16INK4A-ARF locus in LCL 3CHT-E cells (expressing little or no Rb) with HT or without HT in the growth medium. (D) ChIP analysis of H3K4me3 distribution across the p16INK4-ARF locus essentially as described in (C).