Figure 9. Expression of p16^INK4A is increased in CtBPM LCLs relative to revertant and WT LCLs.

(A) E3^CtBP, 3A^CtBP, 3C^CtBP and two rev^CtBP LCLs were harvested 28 days after the infection of primary B cells with the recombinant EBVs. RNA was extracted and the relative levels of CDKN2A transcripts were quantified by qRT-PCR. (B) RNA was extracted from two established E3^CtBP LCLs, two 3A^CtBP LCLs, 3C^CtBP LCL, a revertant (rev^CtBP) and a WT-EBV LCL. The relative levels of p16^INK4A transcripts were quantified by qRT-PCR. (C) Western blot analysis of protein extracts from two E3^CtBP LCLs, two 3A^CtBP LCLs and a 3C^CtBP LCL all established from a single donor. A revertant (rev^CtBP) and a WT-EBV LCL are shown for comparison. Levels of p16^INK4A (p16) are shown relative to γ-tubulin (γ-tub).